A team of scientists from the University of Exeter's flagship Living Systems Institute has shown that stem cells contain no cancer mutations when they are grown in their most primitive or naïve state.

The ground-breaking advances made by the research team should help allay fears surrounding recent controversy about the genetic stability of human embryonic stem cells.

The study is published in the peer-reviewed journal Cell Stem Cell on Monday, December 14th 2020.

Human embryonic stem cells offer great promise for regenerative medicine because they can be turned into every type of cell in our bodies -- such as neurons, heart, pancreatic, and liver cells.

As a result, they represent a significant potential source of cells that could be used to replace those lost through damage or disease.

A major concern, however, had been whether embryonic stem cells acquire cancer-causing mutations.

Recent studies had indicated that human pluripotent stem cells had shown the potential for increased frequency of serious cancer-causing mutations.

However, the new research, led by Senior Research Fellow Dr Ge Guo from the University of Exeter has shown that there is no increased frequency of mutations in cancer-related genes found in these cells.

Analysing RNA-sequencing data from human naïve pluripotent stem calls, the research team found that the actual incidence of cancer-causing mutations were closer to zero.

Dr Guo, who has pioneered research into human naïve embryonic stem cells and is part of the University of Exeter's College of Medicine and Health said: "Our study corrects misinformation in the field and encourages us to continue exploring the potential of naïve stem cells."

Professor Austin Smith co-author of the paper and Director of the Living Systems Institute added: "I am delighted to see Dr Guo launch her team in LSI by publishing these significant results."

Dr Guo's research is focussed on mammalian pluripotent stem cells and cell fate transition during early embryo development.

Key research areas in the lab include understanding the developmental plasticity of human naïve stem cells; Modelling early human embryo development ex vivo by reconstruction of embryo structures; and establishing pluripotent stem cells from various mammalian species and elucidation of shared and distinct gene regulatory features.

The research was funded by the Medical Research Council.

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Journal Reference:

1. Giuliano Giuseppe Stirparo, Austin Smith, Ge Guo. Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells. Cell Stem Cell, 2020; DOI: 10.1016/j.stem.2020.11.014