Most plants (including soybeans, rice, canola, and all trees) are C3 because they fix CO₂ first into a carbohydrate containing three carbon atoms. Corn, sorghum, and sugarcane belong to a special group of plants known as C4, so-called because they first fix CO₂ into a four-carbon carbohydrate during photosynthesis. On average, C4 crops are 60 percent more productive than C3 crops.

When crops are grown in elevated CO₂ that mimic future atmospheric conditions, research shows that C3 crops can become more productive while some experiments suggest that C4 crops would be no more productive in a higher CO₂ world.

"As scientists, we need to think several steps ahead to anticipate what the Earth will look like five to 30 years from now, and how we can design crops to perform well under those conditions," said Charles Pignon, a former postdoctoral researcher at Illinois. "We decided that a literature review and a retrospective analysis of biochemical limitations in photosynthesis would be able to give us some insight into why C4 crops might not respond and how we might alter this."

The literature review, published in Plant, Cell & Environment, was supported by Water Efficient Sorghum Technologies (WEST), a research project that aimed to develop bioenergy crops that produce more biomass with less water, with funding from the Advanced Research Projects Agency-Energy (ARPA-E).

The team assembled a dataset of photosynthesis measurements from 49 C4 species, including the crops that could reveal photosynthetic limitations. The consistent pattern that emerged was that at low CO₂ -- well below what plants would have experienced before the industrial revolution -- C4 photosynthesis was limited by the activity of the enzyme that fixes CO₂. However, at today's CO₂ levels, C4 photosynthesis was limited by the capacity to provide the three-carbon molecule that accepts the fourth CO₂.

"This finding is analogous to a car assembly line where the supply of engines is outpacing the supply of chassis to accept them," said co-author Stephen Long, the Stanley O. Ikenberry Chair Professor of Plant Biology and Crop Sciences. "We need to engineer these plants to better balance their resources in one or both of two-ways."

First, the authors suggest that C4 crops need to cut back on the amount of the enzyme used to fix CO₂ and re-invest the saved resources into making more of the CO₂ acceptor molecule.

Secondly, they need to restrict the supply of CO₂ into the leaf by reducing the number of pores (stomata) on the leaf surface. "Lowering the CO₂ within the leaf would re-optimize the biochemistry, without lowering the rate of photosynthesis, and with fewer stomata, less water would be lost so we are increasing the crop's water use efficiency," Long said.

The WEST project concluded in 2019. These proposed changes to C4 crops are now being pursued through the Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), which is supported by the Department of Energy.

Story Source:

Materials provided by Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign. Original written by Claire Benjamin. Note: Content may be edited for style and length.

Journal Reference:

1. Charles P. Pignon, Stephen P. Long. Retrospective analysis of biochemical limitations to photosynthesis in 49 species: C 4 crops appear still adapted to pre‐industrial atmospheric [ CO 2. Plant, Cell & Environment, 2020; 43 (11): 2606 DOI: 10.1111/pce.13863