Italy's world time-trial champion Filippo Ganna won Saturday's Giro d'Italia 14th stage with Portugal's Joao Almeida tightening his grip on the leader's pink jersey.
Ineos rider Ganna claimed his third stage win in this year's race over 34.1km through Italy's sparkling wine Prosecco region.
Ganna clocked 42min 40sec, with Australian teammate Rohan Dennis second fastest at 26sec and American Brandon McNulty third at 1min 09sec.
Almeida, sixth on the stage at 1min 31 sec, has been leading from the front since stage three.
The Portuguese gained another 16 seconds on second-placed Dutchman Wilco Kelderman, who drops to 56 seconds adrift on the eve of the summit finish in Piancavallo.
UAE Team Emirates' McNulty, 22, moves up to fourth overall, ahead of former two-time Italian race winner Vincenzo Nibali, who dropped to 2min 30 sec in the overall standings.
On Sunday, the Giro returns to altitude in the 15th stage which arrives in the Piancavallo resort after 185km with three second category mountains preceding the final 14.5km climb.
Standings after the 14th stage of the Giro d'Italia on Saturday, a 34km individual time-trial between Conegliano and Valdobbiadene:
1. Filippo Ganna (ITA/Ineos) 42mins 40sec, 2. Rohan Dennis (AUS/INE) at 26sec, 3. Brandon McNulty (USA/UAE) 1:09, 4. Thomas De Gendt (BEL/LOT) 1:11, 5. Josef Cerny (CZE/CCC) 1:16, 6. Joao Almeida (POR/DEC) 1:31, 7. Tanel Kangert (EST/EF1) 1:33, 8. Jonathan Castroviejo (ESP/INE) 1:44, 9. Wilco Kelderman (NED/SUN) 1:47, 10. Jan Tratnik (SLO/BAH) 2:00
Selected
16. Rafal Majka (POL/BOR) 2:37, 22. Pello Bilbao (ESP/BAH) 2:53, 23. Vincenzo Nibali (ITA/TRE) 2:54, 27. Domenico Pozzovivo (ITA/NTT) 3:01, 29. Jakob Fuglsang (DEN/AST) 3:13
Overall standings
1. Joao Almeida (POR/DEC) 54hrs 28mins 9sec, 2. Wilco Kelderman (NED/SUN) at 56sec, 3. Pello Bilbao (ESP/BAH) 2:11, 4. Brandon McNulty (USA/UAE) 2:23, 5. Vincenzo Nibali (ITA/TRE) 2:30, 6. Rafal Majka (POL/BOR) 2:33, 7. Domenico Pozzovivo (ITA/NTT) 2:33, 8. Fausto Masnada (ITA/DEC) 3:11, 9. Patrick Konrad (AUT/BOR) 3:17, 10. Jai Hindley (AUS/SUN) 3:33
Selected
12. Jakob Fuglsang (DEN/AST) 4:08, 35. Diego Ulissi (ITA/UAE Emirates) 49:42, 60. Filippo Ganna (ITA/Ineos) 1.22:33, 70. Peter Sagan (SVK/BOR) 1.33:22