Defects in insulin signaling or insulin resistance in skeletal muscle is important in type 2 diabetes, but with this new approach the researchers have uncovered the potential existence of major signaling defects both inside and outside of the classical insulin signaling cascade.

They found that while a number of proteins involved in the action of insulin were disrupted in cells originating from individuals with type 2 diabetes, the vast majority of changes detected were in proteins with largely unknown roles in metabolism or diabetes.

The findings should provide new perspectives on the mechanisms behind insulin resistance in type 2 diabetes and possibly opportunities for the development of novel therapeutics for the disease.

The research program was led by C. Ronald Kahn MD, Chief Academic Officer, Senior Investigator and Section Head, Integrative Physiology and Metabolism at Joslin Diabetes Center, and Mary K. Iacocca Professor of Medicine at Harvard Medical School. Their full research findings are published in the journal Cell Metabolism.

Focusing on reprogrammed induced pluripotent stem cells, or iPSCs, that were derived from individuals with type 2 diabetes and healthy control subjects, the researchers managed to recreate a cell culture model with many of the features of muscle insulin resistance that occur in humans with diabetes.

They found that the cultured iPSC-derived myoblasts (an early precursor to most muscle cell types) from individuals with type 2 diabetes mirrored many of the impaired molecular responses to insulin and glucose that are seen in humans with diabetes. This included defects in glucose uptake and cellular metabolism.

Using a technique called phosphoproteomics that can measure chemical changes in many thousands of proteins simultaneously, they then found alterations in multiple pathways both within and outside the classical insulin signaling pathway in cells from individuals with type 2 diabetes compared to controls.

This included changes in the way DNA is converted to RNA and then into protein, as well as changes affecting the function and transport of many of these proteins within the cell. On that basis they suggest the work points to a new and previously unrecognized layer of potential targets for developing therapies towards type 2 diabetes.

Lead author Thiago Batista PhD said: "Our findings point to changes on a multiplicity of pathways that are not explained by a single kinase or phosphatase whose function is to regulate cellular signaling. Finding chemical factors that can alone impact the function of multiple kinases and hence signaling pathways would be of great interest in the field."

Senior author C. Ronald Kahn added: "While we are interested in how these newly identified pathways may contribute to disease progression, future research should also aim at better understanding how this might link to both genetic and environmental effects that increase the risks for diabetes. This will open up a whole new range of diagnostic and therapeutic possibilities for this common form of diabetes."

Story Source:

Materials provided by Joslin Diabetes Center. Note: Content may be edited for style and length.

Journal Reference:

1. Thiago M. Batista, Ashok Kumar Jayavelu, Nicolai J. Wewer Albrechtsen, Salvatore Iovino, Jasmin Lebastchi, Hui Pan, Jonathan M. Dreyfuss, Anna Krook, Juleen R. Zierath, Matthias Mann, C. Ronald Kahn. A Cell-Autonomous Signature of Dysregulated Protein Phosphorylation Underlies Muscle Insulin Resistance in Type 2 Diabetes. Cell Metabolism, 2020; DOI: 10.1016/j.cmet.2020.08.007