How understanding the dynamics of yeast prions can shed light on neurodegenerative diseases

In a new study published in PNAS, researchers from Tokyo Institute of Technology and Kanazawa University, led by Prof Hideki Taguchi, set out to dig deeper into prion structures and their mechanisms. They used a modern microscopy technique, called high-speed atomic force microscopy (HS-AFM), developed by Dr. Toshio Ando at Kanazawa University, to visualize the formation of Sup35NM amyloid fibrils in real time. Prof Taguchi explains, "Previous studies used methods that do not allow for the simultaneous, high-resolution assessment of the structures and dynamics involved in Sup35NM oligomerization and fibrillation. To overcome this limitation, we used HS-AFM, which allows the direct visualization of protein molecules in dynamic action at a high resolution."

To begin with, the researchers analyzed Sup35NM monomers using HS-AFM. Their analysis revealed that Sup35NM molecules contained a globular structure with two highly flexible tail-like structures. Then, to observe the oligomeric forms of Sup35NM, monomers incubated in a denaturing solution in controlled conditions, which, in a few hours, led to the formation of oligomers. Further HS-AFM analyses revealed the exact size of these oligomers -- a maximum of 3-4 nm, not growing any longer than this. Thus, the scientists inferred that this size could be an inherent property of oligomers under these conditions.

The researchers then moved on to study the fibril forms of Sup35NM. As opposed to oligomers, fibrils required much a longer incubation time to form, typically 2 to 3 days. In addition, the researchers observed that the fibril elongated smoothly without the attachment of the oligomers. These findings indicated that Sup35NM oligomers are not a prerequisite for the formation of amyloid fibrils, leading the scientists to conclude that oligomers might actually possess cellular functions that are different from those of Sup35NM.

Finally, the researchers studied an interesting phenomenon where oligomers and fibril structures seem to maintain an interspatial distance or "gap." They observed that the trunk of the fibrils has a rigid structure that repels oligomers coming from the sides. Meanwhile, the gap between the tip of the fibril and nearby oligomers is less pronounced because the tip does not have such rigid structures. This helped the scientists understand why Sup35NM amyloid fibrils always grow in a straight direction, without forming any branches.

In sum, these findings provide a detailed account into the structural and functional characteristics of yeast prions -- in their monomeric, oligomeric, and fibril forms. "Our HS-AFM observations of Sup35NM unveiled features of Sup35NM dynamics, providing mechanistic insight into amyloid fibril formation," concludes Prof Taguchi.

Does this new insight into the structure and mechanism of prions bring hope for effective therapies against neurodegenerative diseases in humans? Only time will tell, but this study surely takes a huge step towards that direction.

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Materials provided by Tokyo Institute of Technology. Note: Content may be edited for style and length.

Journal Reference:

1. Hiroki Konno, Takahiro Watanabe-Nakayama, Takayuki Uchihashi, Momoko Okuda, Liwen Zhu, Noriyuki Kodera, Yousuke Kikuchi, Toshio Ando, Hideki Taguchi. Dynamics of oligomer and amyloid fibril formation by yeast prion Sup35 observed by high-speed atomic force microscopy. Proceedings of the National Academy of Sciences, 2020; 201916452 DOI: 10.1073/pnas.1916452117