The service, currently available to Amazon's Prime Now customers in Leeds, Manchester, Birmingham and parts of London, will be rolled out to Glasgow, Newcastle, Liverpool, Sheffield and Portsmouth this year, the companies said.
In future years, Morrisons at Amazon will be expanded to more British cities, they said.
The service allows customers to order a full Morrisons shop online, which is then picked at a local store, and delivered by Amazon, with an option for delivery within one hour of the order being placed in some locations.
(Reporting by Paul Sandle. Editing by Jane Merriman)