Expedeon’s InstantBlue is formulated as a 1X ready-to-use staining reagent. Gels can remain in the stain for weeks without risk of overstaining. Only proteins are stained, resulting in well-defined blue bands on a transparent background. The reduction of background interference results in a good signal-to-noise ratio. Protein bands containing as low as 5 ng per band (BSA) can be detected.
The InstantBlue formulation is non-toxic and does not contain any methanol or acetic acid, so usage and disposal are safe. No methylation or acetylation means that proteins stained using the reagent are compatible with mass spectrometry (MS) analysis and silver staining.
The product is easy to use, with no washing, fixing, heating or destaining steps required. It is also ultrafast, creating visible bands within 15 min or less. It has a shelf life of two years.
Original Article: Expedeon InstantBlue Coomassie protein stain for polyacrylamide gels