Cayman Chemical’s NETosis Imaging Assay Kit provides a simple staining protocol for visualising the process of NETosis kinetically ex vivo and in vitro. A cell-permeable DNA dye allows visualisation of the dynamic changes of the nucleus during the process of NETosis, while a cell-impermeable dye detects the extruded DNA. Combining the fluorescence visualisation of these dyes with brightfield imaging in a high-content platform allows for high-quality differentiation between different modes of cell death. The amount of each reagent provided is sufficient for two 96-well plates.
As an important component of the innate immune system, neutrophil extracellular traps (NETs) can trap and immobilise pathogens, alert other immune cells such as macrophages to clear these pathogens and have direct antimicrobial activity. The process by which NETs are released is a recently described, distinct form of programmed cell death called NETosis. Its mechanisms of action still require a deeper understanding since deviations in NETosis or defects in NET clearance have been implicated in a host of inflammatory disorders and autoimmune diseases, including systemic lupus erythematosus, psoriasis and rheumatoid arthritis.
Original Article: Cayman Chemical NETosis Imaging Assay Kit