The statement by the policy research director of the Institute for American Studies of the foreign ministry accused the US of deliberate provocation over the sanctions on the three officials.
Panyang:
North Korea has condemned the US over its latest sanctions measures, warning the policy could "block the path to denuclearisation on the Korean peninsula forever".
The warning from the North on Sunday came days after the US said it was imposing sanctions on three senior North Korean officials over human rights abuses.
The sanctioned officials include Choe Ryong Hae, who has been considered a right-hand man to leader Kim Jong Un.
In a statement carried by the official KCNA news agency, the North praised President Donald Trump for his efforts to improve relations with Pyongyang, but said the US State Department was "bent on bringing the DPRK-US relations back to the status of last year which was marked by exchanges of fire".
The statement by the policy research director of the Institute for American Studies of the foreign ministry accused the US of "deliberate provocation" over the sanctions on the three officials.
If Washington believes the policy of increased sanctions and pressure would force the North to give up its nuclear weapons, "it will count as greatest miscalculation, and it will block the path to denuclearization on the Korean peninsula forever", the statement added.
At a historic summit in Singapore in June, Trump and Kim signed a vaguely-worded statement on denuclearisation.
But little progress has been made since then, with Washington pushing to maintain sanctions against the North until its "final, fully verified denuclearisation" and Pyongyang condemning US demands as "gangster-like".
In actions required by Congress, the Trump administration said on Monday it would seize any US assets of the three officials over their roles in suppressing freedom of speech.
Such restrictions may have little impact on officials in one of the world's most closed countries but will have a clear symbolic force as North Korea seeks greater acceptance by the United States.