Brazil coach Tite has included Manchester United's Andreas Pereira and Barcelona's Arthur Melo in a vastly changed squad for friendlies against the United States and El Salvador next month.
Teenage Flamengo goalkeeper Hugo Souza was also selected despite having never played for the Rio de Janeiro club's first team.
But there was no place for Manchester City trio Gabriel Jesus, Fernandinho and Ederson while Real Madrid left-back Marcelo and Inter Milan centre-back Miranda were also left out.
Brazil face the United States in New Jersey on September 7 and El Salvador in Washington four days later.
The matches will be the team's first since their 1-2 World Cup quarter-final defeat to Belgium in Russia last month.
Pereira's selection comes just a week after he made his English Premier League (EPL) debut for Manchester United in a deep-lying midfield role.
The 22-year-old is also eligible to play for Belgium, the country of his birth but has publicly stated a preference for Brazil, where both his parents are from.
Tite said he plans to test as many young players as possible ahead of next year's Copa America in Brazil and the start of the 2022 World Cup qualifying tournament later in 2019.
"It's time to take a look at these players," he told a news conference in Rio de Janeiro. "The players who missed out have their importance but now we are taking the opportunity to observe a new generation."
Tite said Brazil's World Cup elimination left a "bad taste" and defended star forward Neymar, who has been heavily criticised for apparent play-acting during the tournament.
"I'm not here to judge or conceptualize behaviours," he said. "I'm not going to give my opinion about what people say, what the media reports. Neymar deserves praise for his behaviour, for his discipline."
The 57-year-old reaffirmed his belief that Neymar is among the world's top three players and insisted that Brazil's No. 10 "has great potential to grow more."
Brazil squad:
Goalkeepers: Alisson (Liverpool), Hugo (Flamengo), Neto (Valencia)
Defenders: Alex Sandro (Juventus), Dede (Cruzeiro), Fabinho (Liverpool), Fagner (Corinthians), Felipe (Porto), Filipe Luis (Atletico Madrid), Marquinhos (Paris St Germain), Thiago Silva (PSG)
Midfielders: Andreas Pereira (Manchester United), Arthur,(Barcelona), Casemiro (Real Madrid), Fred (Manchester United), Lucas Paqueta (Flamengo), Phillipe Coutinho (Barcelona), Renato Augusto (Beijing Guoan)
Forwards: Douglas Costa (Juventus), Roberto Firmino (Liverpool), Neymar (PSG), Pedro (Fluminense), Willian (Chelsea), Everton (Gremio).
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