Of the total 508.17 km of rail track between Mumbai and Ahmedabad, about 21 km is planned to be underground, as per the current alignment of the bullet train project.
One of the entry points to the underground tunnel falls on the land in Vikhroli.
The petition, filed last month, is expected to be heard by a single judge bench of the high court on July 31.
The bullet train project is also facing opposition from farmers in Gujarat and Maharashtra.
Four farmers from Gujarat have petitioned the high court in their state challenging the land acquisition for the project.
The foundation stone for the project was laid by Prime Minister Narendra Modi and his Japanese counterpart Shinzo Abe in September last year in Ahmedabad.
The country's first bullet train will run at a maximum speed of 350 km per hour, covering the stretch in under three hours from the usual seven.
The train will stop at 12 stations out of which four will be in Maharashtra.