The declines in the Asian trading session came despite slim gains on Wall Street, with the move higher in energy shares contributing to gains there. Earlier, stocks stateside had slumped on Monday amid anticipation of further trade measures from the Trump administration against China, although messages from the White House have been conflicting.
"We continue to go in circles on trade policy," said Alex Wolf, senior emerging markets economist at Aberdeen Standard Investments. "[T]he question vexing markets is whether or not this is brinksmanship and can be negotiated, or part of a longer-term strategy aimed at restructuring the U.S.-China economic relationship. The answer, while crucial, is not clear," he said in a note.
Treasury Secretary Steven Mnuchin said on Monday that a Wall Street Journal report on restrictions on Chinese investment in U.S. technology was "fake news," but added that those measures would apply to "all countries" instead of China alone. Despite that, White House economic advisor Peter Navarro told CNBC that there were "no plans" to curb foreign investments.
Uncertainties over trade policy, as well as an escalation in rhetoric in the U.S. trade spat with China in recent weeks, have weighed on market sentiment in Asian markets.
"In the short run, these negotiations and these threats are definitely having an influence. They're raising volatility and they're causing damage to individual sectors ... What's going to really matter is whether or not the trade measures impact the business cycle. Stock prices are basically a child of the business cycle and as long as the business cycle is expanding, then stock markets should be able to continue to rise," John Greenwood, chief economist at Invesco, told CNBC's "Squawk Box."
The declines in China on Wednesday eclipsed the continued gains in oil after prices jumped overnight. Contributing to oil's gains was the U.S. State Department's announcement that companies purchasing Iranian oil would be subject to sanctions if they did not completely slash those imports by November.
U.S. West Texas Intermediate crude futures tacked on 0.51 percent to trade at $70.89 per barrel after crossing the $70 level for the first time in two months overnight. Brent crude futures edged up by 0.8 percent to trade at $76.92.
In currencies, the dollar index, which tracks the greenback against a basket of currencies, mostly held onto overnight gains to trade at 94.612 at 2:50 p.m. HK/SIN. Trade tensions were contributing to near-term strength in the dollar, according to analysts. Against the yen, the dollar softened to trade at 109.80 after trading around the 110 handle on Tuesday.
Meanwhile, the yuan extended its losses to a six-month low on Wednesday. The onshore yuan traded at 6.5930 to the dollar. The People's Bank of China had set the official midpoint at 6.5569 per dollar before the market open. The central bank allows the yuan spot rate to rise and fall a maximum of 2 percent against the dollar relative to the fixing rate.