AFP
Authorities stationed inside and outside the building ralled to block the entrance as raging demonstrators tried to plough through the doors.
The crowd were heard chanting "Save Alfie Evans" as they retreated together outside the hospital.
Tensions rise after the judges at the European Court of Human Rights rejected the appeal to extend Alfie Evan's life-support, revealed today.
An ECHR spokesman said today: "The European Court of Human Rights has today rejected the application submitted by the family of Alfie Evans as inadmissible."
Judges in Strasbourg, France were considering "with urgency" an appeal from Alfie's parents, Tom Evans and Kate James.
Demonstrators are lined up outside the Alder Hey Children's Hospital in Liverpool, protesting at the decision ruled by the court.
PA
Alfie suffers from an undiagnosed neuroligical brain condition and has been at the forefront of the fight against the Liverpool hospital.
This comes after UK judges ruled doctors to stop providing life support treatment to the toddler against his parents wishes, after hearings in the Family Division of the High Court in London and Liverpool.
Alfie's parents, Mr Evans and Mrs James did not succeed in their attempt to convince judges to extend their son's life support at the Supreme Court on Friday.
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In February, Mr Justice Hayden told doctors to cease Alfie's life-support treatment, after hearings in the Family Division of the High Court in London and Liverpool.
Medical staff at Alder Hey told the judge that his treatment "should stop" and Mr Justice Hayden pressed forward on that decision, surrounding evidence showing that further treatment would be in vain.
Mr Evans flew to Rome to meet with religious leader, Pope Francis this week in hopes to transfer his son to a children's hospital in the city to continue palliative care.
Alfie suffers from an unknown neurological condition, leaving him with severe brain injuries that causes him to remain in a "vegetative" state".
His parents have already lost a first round of cases in the High Court, Court of Appeal, European Court of Human Rights and two cases at the Supreme Court.
Campaigners remain outside the Alder Hey hospital in Liverpool, protesting against the courts decisions to cut the toddler's life-support treatment.