Malaysia expelled the North Korean ambassador on Saturday over the death of Kim Jong-nam, the half-brother of North Korean leader Kim Jong-un.
Kang Chol must leave Malaysia within 48 hours, the Foreign Ministry said, reported BBC.
Kim Jong-nam died three weeks ago after two women smeared a deadly chemical over his face at a Kuala Lumpur airport.
Malaysia has not directly blamed North Korea for the attack, but there is suspicion Pyongyang was responsible, according to the report.
North Korea's ambassador Kang was declared "persona non grata" after he said his country "could not trust" Malaysia's handling of the investigation, and that the probe was being interfered with.
Malaysian Foreign Minister Anifah Aman said in a statement that his country had demanded an apology from the envoy for the comments, but none was forthcoming.
"Malaysia will react strongly against any insults made against it or any attempt to tarnish its reputation," Anifah said.
Malaysia was one of the very few countries which had relatively friendly relations with North Korea.
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